The Relationship of Fever Onset with NS1 Examination Results, IgM, and IgG in Dengue Hemorrhagic Fever Patients

Engla Merizka, Assha Luthfianie Alifah, Sri Wahyuni Handayani, Aninda Dinar Widiantari, Farida Murtiani

Abstract


Fever onset represents the average number of days from the beginning of fever symptoms to the time when IgM and IgG examinations are conducted. Determining the right timing for these examinations can significantly impact their accuracy. In November 2021, there was a notable surge in cases of Dengue Hemorrhagic Fever at the Karawang Health Center, Sukabumi. This study aimed to investigate the relationship between the duration of fever and the results of dengue antigen and antibody tests. The research employed a descriptive analysis with a cross-sectional approach, and a sample of 50 individuals was gathered through consecutive sampling. The data was then analyzed using the Chi-Square test. The study revealed a positive correlation between the duration of fever and the outcomes of the NS1 and IgG Dengue examinations (p<0.05), highlighting the significance of timing when conducting these tests. NS1 was predominantly detected within the first three days of fever, while IgG showed reactivity after more than five days of fever. These findings underscore the importance of timing when conducting these tests and interpreting their results.


Keywords


dengue hemorrhagic fever; duration of fever; IgG dengue; IgM dengue; NS1

Full Text:

PDF

References


Agarwal, A., Jain, R. K., Chaurasia, D., & Biswas, D. (2022). Determining the Optimum Cut-Off IgM/ IgG Ratio for Predicting Secondary Dengue Infections: An Observational Hospital Based Study from Central India. Indian Journal of Medical Microbiology, 40(4), 492–495. https://doi.org/10.1016/j.ijmmb.2022.08.015

Ahmed, N. H., & Broor, S. (2014). Comparison of NS1 Antigen Detection ELISA, Real Time RT-PCR and Virus Isolation for Rapid Diagnosis of Dengue Infection in Acute Phase. J Vector Borne Dis, 51, 194–199.

Anwar, F., Ahmad, S., Haroon, M., Ul Haq, I., Ullah Khan, H., Khyber Teaching Hospital Peshawar, D., Jawad Khan, P., Ullah, S., Ali Shah, I., Iftikhar Ali Shah, C., & Khan, J. (2019). Dengue Virus Epidemics: A Recent Report of 2017 from District Mardan, Khyber Pakhtunkhwa Province, Pakistan. International Journal of Mosquito Research, 46(1), 46–49.

Apriliana, E., Tjiptaningrum, A., & Prayoga, M. J. (2019). The Relationship between Non-Structural 1 (NS1) Antigen Test Result to Symptoms, Clinical Signs and Platelets Count on Patients with Suspected Dengue Infection in Urip Sumoharjo Hospital. J Agromedicine, 6(1), 30–37.

Duong, V., Ly, S., Lorn Try, P., Tuiskunen, A., Ong, S., Chroeung, N., Lundkvist, A., Leparc-Goffart, I., Deubel, V., Vong, S., & Buchy, P. (2011). Clinical and Virological Factors Influencing the Performance of a NS1

Antigen-Capture Assay and Potential Use as a Marker of Dengue Disease Severity. PLoS Neglected Tropical Diseases, 5(7), e1244. https://doi.org/10.1371/journal.pntd.0001244

Ghosh, A., Sukla, S., Nath, H., Saha, R., De, A., & Biswas, S. (2022). Non-Structural Protein 1 (NS1) Variants from Dengue Virus Clinical Samples Revealed Mutations That Influence NS1 Production and Secretion. European Journal of Clinical Microbiology & Infectious Diseases, 41(5), 803–814. https://doi.org/10.1007/s10096-022-04441-4

Gusti, I., Dwi Mahasurya, A., Agung, A., Lestari, W., Putu, W., & Yasa, S. (2017). Gambaran Pemeriksaan Serologi IgM-IgG Antidengue Pasien Treinfeksi Virus Dengue Di Rumah Sakit Surya Husada Denpasar Bali pada Periode Desember 2013 sampai Mei 2014. E-Jurnal Medika Udayana, 6(1). https://ojs.unud.ac.id/index.php/eum/article/view/28895

Khairinisa, G., Alamanda, C., & Permatasari. Pelita. (2018). Deteksi Antigen Non-Struktural 1 (NS1) pada Pasien yang Diduga Terinfeksi Dengue. Prosiding Pertemuan Ilmiah Nasional Penelitian & Pengabdian Masyarakat (PINLITAMAS 1), 1–1.

Kurnia, N. (2022). Pemeriksaan Serologis IgG-IgM pada Pasien Demam Berdarah Dengue di RSUD Dr. Chasan Basoeri Ternate Periode Oktober - Desember 2021. JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan), 7(2), 117. https://doi.org/10.30829/jumantik.v7i2.11294

Lei, Z., Feng, Y., & Yanli, W. (2022). Detection of Specific Anti-Dengue IgM and IgG Antibodies Differentiate Primary from Secondary Infection. Health Science Journal, 16(11). https://doi.org/10.36648/1791

Liang, D., Juan, S. U., & Hong, X. (2021). Evaluation of Dengue NS1 Antigen, Viral RNA, And IgM Antibody for Early Laboratory Diagnosis of Single Specimens from Clinically Suspected Patients During The 2014 Dengue Epidemic in Guangdong, China. Chinese Journal of Public Health, 37(3). https://doi.org/10.11847/zgggws1127062

Libraty, D. H., Young, P. R., Pickering, D., Endy, T. P., Kalayanarooj, S., Green, S., Vaughn, D. W., Nisalak, A., Ennis, F. A., & Rothman, A. L. (2002). High Circulating Levels of the Dengue Virus Nonstructural Protein NS1 Early in Dengue Illness Correlate with the Development of Dengue Hemorrhagic Fever. The Journal of Infectious Diseases, 186(8), 1165–

https://doi.org/10.1086/343813

Luvira, V., Thawornkuno, C., Lawpoolsri, S., Thippornchai, N., Duangdee, C., Ngamprasertchai, T., & Leaungwutiwong, P. (2023). Diagnostic Performance of Dengue NS1 and Antibodies by Serum Concentration Technique. Tropical Medicine and Infectious Disease, 8(2), 117. https://doi.org/10.3390/tropicalmed8020117

Masykur, F. abdul. (2022). Hubungan Antara Lama Demam dengan Hasil Pemeriksaan Profil Darah pada Pasien Demam Berdarah Dengue. Jurnal Ilmu Medis Indonesia, 1(2), 53–58. https://doi.org/10.35912/jimi.v1i2.912

Mata, V. E., Andrade, C. A. F. de, Passos, S. R. L., Hökerberg, Y. H. M., Fukuoka, L. V. B., & Silva, S. A. da. (2020). Rapid Immunochromatographic Tests for The Diagnosis of Dengue: A Systematic Review and Meta-Analysis. Cadernos de Saúde Pública, 36(6). https://doi.org/10.1590/0102-311x00225618

Mora-Cárdenas, E., Aloise, C., Faoro, V., Knap Gašper, N., Korva, M., Caracciolo, I., D’Agaro, P., Avšič-Županc, T., & Marcello, A. (2020). Comparative Specificity and Sensitivity of NS1-Based Serological Assays for The Detection of Flavivirus Immune Response. PLOS Neglected Tropical Diseases, 14(1), e0008039.

https://doi.org/10.1371/journal.pntd.0008039

Ndiaye, O., Woolston, K., Gaye, A., Loucoubar, C., Cocozza, M., Fall, C., Dia, F., Adams, E. R., Samb, M., Camara, D., Sadio, B. D., Diagne, C. T., Weidmann, M., Faye, O., Fitchett, J. R. A., Sall, A. A., & Diagne, C. T. (2023). Laboratory Evaluation and Field Testing of Dengue NS1 and IgM/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal. Viruses, 15(4), 904. https://doi.org/10.3390/v15040904

Nguyet, N. M., Hien, T. T., Simmons, C. P., Farrar, J., Van Vinh Chau, N., Wills, B., Thuy, T. T., Anders, K. L., Hung, N. T., & Lien, L. B. (2011). Epidemiological Factors Associated with Dengue Shock Syndrome and Mortality in Hospitalized Dengue Patients in Ho Chi Minh City, Vietnam. The American Journal of Tropical Medicine and Hygiene, 84(1), 127–134. https://doi.org/10.4269/ajtmh.2011.10-0476

Ngwe Tun, M. M., Thant, K. Z., Inoue, S., Kurosawa, Y., Lwin, Y. Y., Lin, S., Aye, K. T., Thet Khin, P., Myint, T., Htwe, K., Mapua, C. A., Natividad, F. F., Hirayama, K., & Morita, K. (2013). Serological Characterization of Dengue Virus Infections Observed Among Dengue Hemorrhagic Fever/Dengue Shock Syndrome Cases in Upper Myanmar. Journal of Medical Virology, 85(7), 1258–1266. https://doi.org/10.1002/jmv.23577

Pandaibesi, R. (2017). Hubungan Pengetahuan, Sikap dan Perilaku Masyarakat dengan Kejadian Demam Berdarah di Kecamatan Medan Sunggal. https://repositori.usu.ac.id/handle/123456789/3473

Permatasari, D. Y., Ramaningrum, G., & Novtasari, A. (2015). Hubungan Status Gizi, Umur, dan Jenis Kelamin dengan Derajat Infeksi Dengue pada Anak. Jurnal Kedokteran Muhammadiyah, 2(1), 24–28.

Pramatama, S., Wijayanti, M., Dewi, B. E., Anugrha, H. W., Goentoro, P. L., & Sudiro, M. (2020). Efficacy of Ns1 Antigen Detection for Early Dengue Infection Diagnosis in Indonesia (Vol. 51, Issue 1).

Puspitasari, D., Dewi, S., & Aryati, A. (2017). The Relationship of Ns1 Antigen Profi Le and Days of Illness in Children with Dengue Virus Infection. Jurnal Ners, 8(1), 41–46. https://doi.org/10.20473/jn.v8i1.3867

Rahmi, F., Hadi, D., Fransiska, M., & Kustanto, D. R. (2019). Analisis Epidemiologi Penyakit Demam Berdarah Dengue Melalui Pendekatan Temporal dan Hubungannya Dengan Faktor Iklim Di Kota Padang Tahun 2014-2017. Jurnal Kesehatan, 10(1), 22.

https://doi.org/10.35730/JK.V10I1.373

Santos, C. da C., Santos, P. C. M., Rocha, K. L. S., Thomasini, R. L., de Oliveira, D. B., Franco, D. L., & Ferreira, L. F. (2020). A New Tool for Dengue Virus Diagnosis: Optimization and Detection of Anti-NS1 Antibodies in Serum Samples By Impedimetric Transducers. Microchemical Journal, 154, 104544. https://doi.org/10.1016/j.microc.2019.104544

Satriadi, R., Parinding, J. T., Tejoyuwono, A. A. T., & Natalia, D. (2021). Hubungan antara Lama Demam dengan Hasil Pemeriksaan Kualitatif IgM dan IgG pada Pasien Demam Berdarah Dengue (DBD) di RSUD Sultan Syarif Mohamad Alkadrie tahun 2018. Jurnal Vektor Penyakit, 15(1), 11–16. https://doi.org/10.22435/vektorp.v15i1.4125

Sharada, T., Raghunath, P., & Suma, N. (2021). Detection of NS1 Antigen, IgM And IgG Antibodies Using A Commercial Dengue Rapid Test Kit for The Diagnosis of Dengue Infection in Patients with Acute Febrile Illness. Indian Journal of Microbiology Research, 8(3), 235–238. https://doi.org/10.18231/j.ijmr.2021.048

Singhal, T., & Kothari, V. (2020). Clinical and Laboratory Profile of Fatal Dengue Cases at a Tertiary Care Private Hospital in Mumbai, India. The American Journal of Tropical Medicine and Hygiene, 103(3), 1223–1227. https://doi.org/10.4269/ajtmh.19-0425

Soedarmo SS. (2005). Demam Berdarah (Dengue) pada Anak (2nd ed.). Universitas Indonesia.

Sumampouw, O. J. (2020). Epidemiologi Demam Berdarah Dengue di Kabupaten Minahasa Sulawesi Utara. Sam Ratulangi Journal of Public Health, 1(1), 001. https://doi.org/10.35801/srjoph.v1i1.27272

Van de Weg, C. A. M., van den Ham, H.-J., Bijl, M. A., Anfasa, F., Zaaraoui-Boutahar, F., Dewi, B. E., Nainggolan, L., van IJcken, W. F. J., Osterhaus, A. D. M. E., Martina, B. E. E., van Gorp, E. C. M., & Andeweg, A. C. (2015). Time Since Onset of Disease and Individual Clinical Markers Associate with Transcriptional Changes In Uncomplicated Dengue. PLOS Neglected Tropical Diseases, 9(3), e0003522. https://doi.org/10.1371/journal.pntd.0003522

Zaidi, M. B., Cedillo-Barron, L., González y Almeida, M. E., Garcia-Cordero, J., Campos, F. D., Namorado-Tonix, K., & Perez, F. (2020). Serological Tests Reveal Significant Cross-Reactive Human Antibody Responses to Zika and Dengue Viruses in The Mexican Population. Acta Tropica, 201, 105201. https://doi.org/10.1016/j.actatropica.2019.105201




DOI: http://dx.doi.org/10.30742/jikw.v12i2.2645

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Engla Merizka, Assha Luthfianie Alifah, Sri Wahyuni Handayani, Aninda Dinar Widiantari, Farida Murtiani

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Jurnal Ilmiah Kedokteran Wijaya Kusuma is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License