The Effect of Melinjo Peel Extract in Activity of Lipoprotein Lipase Enzym of The Rats Fed a Hypercholesterolemia Diet

Athira Demitri

Abstract


The intake of saturated fat and cholesterol that comes from food digested in the intestine resulting in free fatty acids, triglycerides, phospholipids, and cholesterol. Triglyceride levels increased can be caused by presence of impaired lipoprotein lipase (LPL) enzyme activity. LPL activity can be increased by flavonoid in plant, like Melinjo peel. The purpose of this study is to measure LPL enzyme activity before and after being given extract of Melinjo peel treatment. This research use true experimental, with study design of pretest posttest control group design. Hypercholesterolemic diet given to rats by oral gavage as much as 1,8 grams for 14 days. Melinjo peel extract were given by oral gavage for 14 days after the hypercholesterolemic diet is given. Statistical analysis used Paired Sample T-Test to compare lipoprotein lipase activity before and after treatment. Then, used MANOVA (Multivariate Analyses of Variance) to see the difference of lipoprotein lipase activity in each group after treatment.

Based on statistical analysis showed that there were differences of lipoprotein lipase activity in the hypercholesterolemic diet group, HD+54.15, HD+108.30, and HD+216.60 before and after treatment significant (p. < 0.05). The activity of lipoprotein lipase in the normal diet group compared with the hypercholesterolemic and HD+216.60 group showed a significant difference (p. < 0.05). Melinjo peel extract can increased activity of lipoprotein lipase enzym after treatment, those can be due to the flavonoid in Melinjo peel extract.


Keywords


Hypercholesterolemia, Lipoprotein Lipase, Gnetum gnemon peel, Flavonoid

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References


Beekmann K, Rubio L, DE Haan LHJ, Actis-Goretta L, van der Burg B, et al, 2015. The Effect of Quercetin and Kaempfrol Aglycones and Glucoronides on Peroxisome Proliferator-Activated Receptor-Gamma (PPAR). Journal Food and Function. 6: 1098 – 1107

Fan C, Yan J, Qian Y, Wo X, Gao L, 2006. Regulation of Lipoprotein Lipase Expression by Effect of Hawthorn Flavonoids on Peroxisome Proliferator Response Element Pathway. Journal of Pharmacological Sciences, 100 (1): 51 – 58

Geldenhuys WJ, Lin L, Darvesh AS, and Sardana P, 2017. Emerging Strategies of Targeting Lipoprotein Lipase for Metabolic and Cardiovascular Diseases. Drug Discovery Today, 22 (2): 352 – 365

Handayani D, Am A, Soeadmadji DW, Widodo MA, 2003. Lipoprotein Lipase Enzyme as an Alternative Test to Diagnose Lipid Metabolism Disturbances in Diabetes Type 2. Maj. Kedok. Unibraw, XIX (2): 1 - 8

Kersten S, 2014. Physiological Regulation of Lipoprotein Lipase. Review Article. Biochimica et Biophysica Acta, 1841 (7): 919 - 933

Kozlowska A dan Szostak-Węgierek D, 2014. Flavonoids - Food Sources and Health Benefits. National Institute of Public Health-National Institute of Hygiene, 65 (2): 79 – 85

Olivecrona G, 2016. Role of Lipoprotein Lipase in Lipid Metabolism. Current Opinion in Lipidology. 27 (3): 233 – 241

Ooi EMM, Watts G F, Ng TWK, and Barrett PHR, 2015. Effect of Dietary Fatty Acids on Human Lipoprotein Metabolism: A Comprehensive Update. Nutrients, 7 (6): 4416 – 4425

Saputra S, 2015. Pengaruh Pemberian Yoghurt Sinbiotik Tanpa Lemak dengan Penambahan Tepung Gembili (Dioscorea Esculenta) Terhadap Kadar Kolesterol Total Tikus Hiperkolesterolemia. Artikel Penelitian. Prodi Studi Ilmu Gizi Fakultas Kedokteran Universitas Diponegoro, Semarang.

Setiawan DI, Tjahyono K, dan Afifah DN, 2016. Pemberian Kecambah Kacang Kedelai Terhadap Kadar Malondialdehid (MDA) dan Superoxide Dismutase (SOD) Tikus Sprague Dawley Hiperkolesterolemia. Jurnal Gizi Klinik Indonesia, 13 (1): 20 – 26




DOI: http://dx.doi.org/10.30742/jikw.v7i2.433

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Jurnal Ilmiah Kedokteran Wijaya Kusuma 

ISSN: 1978-2071(print); 2580-5967 (online)
 
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