Apoptosis Sel Otot Rangka dan Perubahan Berat Badan pada Tikus Diabetes yang Diberi Ekstrak Umbi Bawang Dayak (Eleutherine palmifolia L., Merr)

Lailia Dwi Kusuma Wardhani, Olan Rahayu Puji Astuti


This study was undertaken to investigate the antidiabetic and antioxidant of bawang dayak bulbs (Eleutherine palmifolia L., Merr) extract (BDBE) on skeletal muscle cell apoptosis and body weight change in diabetic rat. Single doses alloxan 120 mg/kgBW were administered intraperitoneally to get diabetic rat. Twenty four male Wistar rat of three months old were used in this study. Rat were devided into six groups: negative control group (were not diabetic and treated) (K0), positive control group (were diabetic and treated CMC-Na) (K1), drug control group (were diabetic and administrated metformin as a standard drug) (K2), BDBE dosed 200 (P1), 400 (P2), and 800 mg/kgBW (P3). The treatment was conducted for 14 days. Hypoglycemic effect and body weight measured of all mice was determined at day 7 and 14 of treatment. At the end of research, all of rat were sacrificed and m. gastrocnemius were collected for apoptosis analysis by TUNEL staining and atrophy analysis by Hematoxilin-Eosin staining. The result of this study showed that BDBE decreased skeletal muscle cell apoptosis and mantained the skeletal muscle fiber diameter (atrophy) and body weight change in diabetes rat.


bawang dayak (Eleutherine palmifolia L., Merr), diabetes mellitus, skeletal muscle cell apoptosis, skeletal muscle fiber diameter (atrophy), body weight change

Full Text:



Agusti AC, Jaume S, Cristina M, Bernat T, Ernest S et al, 2002. Skeletal Muscle Apoptosis and Weight Loss in Chronic Obstructive Pulmonary Disease. Am. J. Respir. Crlt. Med. 166:485-489

Ashida H, Furuyashiki T, Nagayasu H, Bessho H, Sakakibara H et al, 2004. Anti-Obesity Actions Of Green Tea: Possible Involvements In Modulation Of The Glucose Uptake System And Suppression Of The Adipogenesis-Related Transcription Factors. Biofactors. 22: 135-140

Bassil MS, and Gougeon R, 2013. Muscle Protein Anabolism In Type 2 Diabetes. Curr.Opin.Clin.Nutr.Metab.Care. 16: 83-88

Cohen S, Nathan JA, and Goldberg AL, 2015. Muscle Wasting In Disease: Molecular Mechanisms And Promising Therapies. Nature. Rev. Drug. Discov. 14: 58-74

Cotran RS, Kumar V, Collius T, 2012. Robbins Pathologic Basic of Disease. 9th ed. Philadelphia: WB Saunders Company

Ebert SM, Zougbi A, Bochine S, and Adams C, 2019. Skeletal Muscle Atrophy: Discovery of mechanism and Potencial Therapies. American Physiological Society Journal .Vol 34(4).

Febrinda AE, 2014. Potensi Antioksidan dan Antidiabetik Ekstrak Air dan Etanol Umbi Bawang Dayak (Eleutherine Palmifolia) secara In Vitro dan In Vivo. Tesis. Fakultas Teknlogi Pertanian, Institut Pertanian Bogor, Bogor.

Ieyama T, Gunawan-Puteri MDPT, and Kawabata J, 2011. a-Glucosidase Inhibitors from the Bulb of Eleutherine americana. J. Food Chemistry .128: 308–311

International Diabetes Federation. 2015. IDFDiabetes Atlas 7th Edition. Brussels:International Diabetes Federation.http://www.diabetes atlas.org/. [Sitasi: 18 November 2016]

Kamalakkannan N, and Prince PSM, 2006. Antihyperglycaemic And Antioxidant Effect Of Rutin, A Polyphenolic Flavonoid, In Streptozotocin Induced Diabetic Wistar Rats. Bas.Clinic.Pharmacol.Toxicol. 98(1): 97-103

Khan HA, 1971. Comparison of Methods for Measurement of Fiber Diameter in Skeletal Muscle. Master’s Thesis. Kansas State University: 5-7

Khorramabadi KM, Talebi AR, Sarchehmeh A, and Mirjalili A, 2019. Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice. International Journal of Reproductive BioMedicine .17(2): 127-134

Lee J, Li Y, Li C, and Li D, 2011. Natural products and body weight control. American Journal of the Medical Sciences. 3(1): 13-19.

Lolok N, Mashar HM, Annah I, Saleh A, Yuliastri WO et al, 2019. Antidiabetic Effect Of The Combination Of Garlic Peel Extract (Allium sativum) And Onion Peel (Allium cepa) In Rats WithOralGlucose Tolerance Method. Research Joournal of Pharmacy and Technology. Fakultas Farmasi STIKES Mandala Waluya, Kendari.

Lundell L, Massart, Julie, Ali A, Anna K et al, 2019. Regulation of glucose uptake and inflammation markers by FOXO1 and FOXO3 in skeletal muscle. Molecular Metabolism. 20.

Maksoed A, Afaf DA, Mustafa Y, Reem S, and Elhariif M, 2018. Biochemical and Genetical Evaluation of Ameliorative Role of Liquorice Extract on Experimentallly Induced hepatitis. International Journal of Current Research. 10(9)

Narici MV, and de Boer MD, 2011. Disuse of the musculo-skeletal system in space and on earth. European Journal of Applied Physiology. 111: 403-420.

Rains JL, and Jain SK, 2011. Oxidative Stress, Insulin Signaling, and Diabetes. Free. Radic. Biol. Med. 50(5): 567-575

Salvesen GS, Dixit VM, 1999. Caspase Activation: The Induced Proximity Model. Proc. Natl. Acad. Sci. USA. 96: 10964-10967.

O’Neill B, Gourav B, Christie MP, Negan T, Palbo AS et al, 2019. FoxO Transcription Factors areCritical Regulators of Diabetes-related muscle Atrophy. American Diabetes Association. 68(3): 556-570

Rohilla A, and Ali S, 2012. Alloxan Induced Diabetes; Mechanism and Effects. International Journal of Research in Pharmaceutical and Biomedical Sciences. Vol 3(2): 819-823.

Shaw JE, Sicree RA, and Zimmet PZ, 2010. Global Estimates of The Prevalence of Diabetes for 2010 and 2030. Diabetes Research and Clinical Practice. 87: 4-14.

Sasakawa Y, Akari K, Michiyo A, Kaori Y, Mayumi N et al, 2017. The Anti-obesity and Anti-inflammatory Effects of “LICONINE”, an Extraxt of Glycyrrhiza Uralensis, on Diet=induced Obese Mice and 3T3-L1 Mouse Adipocytes. Journal of Food and Nutrition Research.5(10): 781-788

Wang H, Cui Y, Zhao C, 2010. Flavonoids of the genus Iris (Iridaceae). MiniRev. Med. Chem. 10: 63

Zabielski P, Blachnio-Zabielska A, Lanza IR, et al, 2016. Altered Skeletal Muscle Mitochondrial Proteome As the Basis of Disruption of Mitochndrial Function in Diabetic Mice. Am. J. Physiol. Endocrinol. Metab. 65: 561–573

Zhang P, Zhang X, Brown J, Vistisen D, Sicree R et al, 2010. Global Healthcare Expenditure on Diabets for 2010 and 2030. Diabets. Research. Clinicl. Practise. 87(3): 291-302

Zheng B, Ohkawa SLiH, Roberts-Wilson TK, and Price SR, 2010. FOXO3a Mediates Signaling Crosstalk That Coordinates Ubiquitin and Atrogin-1/Mafbx Expression During Glucocorticoid induced Skeletal Muscle Atrophy. FASEB J. 24: 2660-2669

DOI: http://dx.doi.org/10.30742/jikw.v9i2.807


  • There are currently no refbacks.

Copyright (c) 2020 Lailia Dwi Kusuma Wardhani,Olan Rahayu Puji Astuti1

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Jurnal Ilmiah Kedokteran Wijaya Kusuma is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License