Pengaruh Pemberian Ekstrak Kayu Manis Terhadap Gambaran Histopatologi Ginjal Pada Tikus Putih Jantan Yang Diinduksi Parasetamol

Rachma Eka Rani, Fitri Handajani, Eva Pravitasari Nefertiti

Abstract


Abstrak

Latar Belakang : Kayu manis (Cinnamomun burmannii) mengandung flavonoid dan sinamaldehid yang berguna sebagai antioksidan dan bersifat renal protektor. Parasetamol berguna sebagai antipiretik dan analgesik. Parasetamol dimetabolisme oleh sitokrom P450 di sel hepar membentuk glukoronida, sulfat, dan NAPQI. NAPQI merupakan hasil metabolit yang sangat reaktif dan mengakibatkan stres oksidatif. Penelitian ini bertujuan untuk engetahui efek pemberian ekstrak kayu manis  yang dapat mencegah kerusakan sel tubulus ginjal tikus putih jantan yang diinduksi parasetamol.

Metode :  30 ekor tikus yang dibagi menjadi 3 kelompok; (1) kelompok kontrol negatif tanpa perlakuan, (2) kelompok kontrol positif yang diinduksi parasetamol dosis tunggal 1750 mg/kgBB pada hari ke 14, (3) kelompok perlakuan  yang diberi ekstrak kayu manis dosis 400 mg/kgBB selama 14 hari lalu diinduksi parasetamol dosis tunggal 1750 mg/kgBB pada hari ke 14. Pada hari ke 17  hewan coba dikorbankan, dilakukan pengambilan ginjal dan dipemeriksa secara mikroskopik. Selanjutnya dilakukan uji Kruskal-Wallis dan uji Mann-Whitney U.

Hasil : Uji  Kruskal-Wallis menunjukkan terdapat perbedaan derajat kerusakan ginjal yang signifikan. Uji Mann-Whitney U menunjukkan perbedaan yang bermakna antara kelompok kontrol negatif dan kelompok kontrol positif p=0,001 (p<a); kelompok kontrol negatif dan kelompok perlakuan p=0,001 (p<a); juga antara kelompok kontrol positif dan kelompok perlakuan p=0,001 (p<a).

Kesimpulan : Terdapat pengaruh pemberian ekstrak kayu manis gambaran histopatologi ginjal  tikus putih jantan yang diinduksi parasetamol.

Kata Kunci : Ekstrak kayu manis (Cinnamomum burmannii), parasetamol, gambaran histopatologi ginjal

 

Abstract

Background: Cinnamon (Cinnamomum burmannii) contains flavonoids and cinnamaldehyde which are useful as antioxidants and renal protectors. Paracetamol is useful as an antipyretic and analgesic. Paracetamol is metabolized by cytochrome P450 in liver cells to form glucuronide, sulfate, and NAPQI. NAPQI is the result of highly reactive metabolites and cause oxidative stress. This study aim to know the effect of cinnamon extract  which can prevent damage of renal tubular cell rats  male induced by paracetamol.

Method: 30  rats used and divided into 3 groups (1) untreated group, (2) rats which were induced by single dose paracetamol 1750 mg/kgBW on 14th day, and  (3) rats were which given cinnamon extract dose 400 mg/kgBW for 14 days then induced single dose paracetamol 1750 mg/kgBW on day 14th. On the 17th day the kidneys are taken and microscopic examination is performed. Data analysis uses the Kruskal-Wallis test and the Mann-Whitney U test.

Result: The Kruskal-Wallis test showed a significant difference in the degree of kidney damage. The Mann-Whitney U test showed a significant difference between the negative control group and the positive control group p=0.001 (p <); negative control group and the treatment group p=0.001 (p <); also between the positive control group and the treatment group p=0.001 (p <).

Conclusion: There is an effect of administration of cinnamon extract  on the histopathological picture of the kidney of white rats male induced by paracetamol.

Keyword:  Cinnamon extract (Cinnamomum burmannii), paracetamol, renal histopathology.

 


Full Text:

PDF

References


Abdeen, A. et al. (2019) “Protective effect of cinnamon against acetaminophen-mediated cellular damage and apoptosis in renal tissue,” Environmental Science and Pollution Research. Environmental Science and Pollution Research, 26(1), hal. 240–249. doi: 10.1007/s11356-018-3553-2.

Brunton, L. L. (2008) Goodman & Gilman Manual Farmakologi dan Terapi.

Cook, S. F. et al. (2016) “Population Pharmacokinetics of Intravenous Paracetamol (Acetaminophen) in Preterm and Term Neonates: Model Development and External Evaluation,” Clinical Pharmacokinetics. Springer International Publishing, 55(1), hal. 107–119. doi: 10.1007/s40262-015-0301-3.

Dirjen POM RI (2009) Farmakope Indonesia edisi IV, Departemen Kesehatan Republik Indonesia. doi: 10.1590/S1984-82502011000100002.

Ferry, Y. (2013) “( Cinnamomum Burmanii L ) DI INDONESIA DEVELOPMENT PROSPECTS OF CINNAMON PLANT ( Cinnamomum Burmanii L ) IN INDONESIA,” Sirinov, 1(1), hal. 11–20.

Godoy-Santos, A. L., Rammelt, S. dan Zoboli, A. C. (2017) Clinical anatomy, Foot and Ankle Sports Orthopaedics. doi: 10.1007/978-3-319-15735-1_1.

Greenstein Gary (2007) “The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals (14th edition),” Reference Reviews. Emerald Group Publishing Limited, 21(6), hal. 40. doi: 10.1108/09504120710775534.

Handajani, F (2019) OKSIDAN DAN ANTIOKSIDAN PADA BEBERAPA PENYAKIT DAN PROSES PENUAAN. 1 ed. Diedit oleh Fitri Handajani. Surabaya: Zifatama Jawara. Tersedia pada: https://books.google.co.id/books?id=H9jFDwAAQBAJ.

Harvey, R. A. (2012) Lippincott’s Illustrated Reviews: Pharmacology 5th edition.

Katzung, B. G. dan Trevor, A. J. (2015) T H a R I N D E T I.

Kumar (2012) Robbins Basic Pathology, 9th ed, Elsevier. doi: 10.1007/s13398-014-0173-7.2.

Lee, H. S. (2002) “Inhibitory activity of Cinnamomum cassia bark-derived component against rat lens aldose reductase,” Journal of Pharmacy and Pharmaceutical Sciences, 5(3), hal. 226–230.

Luft, F. C. et al. (1984) “Ceftazidime nephrotoxicity in rats,” Antimicrobial Agents and Chemotherapy, 25(4), hal. 513–514. doi: 10.1128/AAC.25.4.513.

Masturoh, Imas; T, N. A. (2018) metodologi penelitian kesehatan.

Mitić-Zlatković et al. (1998) “Kidney extopeptidases in gentamicin and mercuric chloride-induced acute renal failure,” Cellular Physiology and Biochemistry, 8(5), hal. 278–284. doi: 10.1159/000016289.

Peter, K. V. (2002) “Handbook of herbs and spices,” I.

Radak, Z. et al. (2008) “Exercise, oxidative stress and hormesis,” Ageing Research Reviews, 7(1), hal. 34–42. doi: 10.1016/j.arr.2007.04.004.

Rodwell, V. W. dan Bender, D. A. (2015) e t i n d Illustrated Biochemistry. doi: 10.1021/acs.langmuir.6b03449.

Scott, G. B. D. (1988) Functional Histology - a Text and Colour Atlas, Postgraduate Medical Journal. doi: 10.1136/pgmj.64.747.93.

Sherwood, L. (2010) Introduction to human physiology.

Sjödin, B., Westing, Y. H. dan Apple, F. S. (1990) “Biochemical Mechanisms for Oxygen Free Radical Formation During Exercise,” Sports Medicine, 10(4), hal. 236–254. doi: 10.2165/00007256-199010040-00003.

Sugiharto, W. (2018) “Hang tuah medical journal,” 15, hal. 112–132.

Vangalapati, M. et al. (2012) “A review on pharmacological activities and clinical effects of Cinnamon species,” Research Journal of Pharmaceutical, Biological and Chemical Sciences, 3(1), hal. 653–663.

Widiyanto, I. et al. (2013) EKSTRAKSI OLEORESIN KAYU MANIS (Cinnamomum burmannii) : OPTIMASI RENDEMEN DAN PENGUJIAN KARAKTERISTIK MUTU THE EXTRACTION OF CINNAMON (Cinnamomum burmannii) OLEORESIN: THE YIELD OPTIMIZATION AND THE EXAMINATION OF QUALITY CHARACTERISTICS, Jurnal Teknologi Hasil Pertanian.

Yousef, M. I. et al. (2010) “Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat,” Food and Chemical Toxicology. Elsevier Ltd, 48(11), hal. 3246–3261. doi: 10.1016/j.fct.2010.08.034.




DOI: http://dx.doi.org/10.30742/jikw.v10i2.1166

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Rachma Eka Rani, Fitri Handajani, Eva Pravitasari Nefertiti

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 
 

ISSN (Print): 1978-2071

E-ISSN (Online): 2580-5967

 

Editorial Office

Fakultas Kedokteran Universitas Wijaya Kusuma Surabaya
Jl. Dukuh Kupang XXV no 54 Surabaya, Indonesia

Gedung C lantai 2 (Ruang C216)
Telp. (+62) 31-5686531

Fax.  (+62) 31-5686531

email: jurnalkedokteranuwks@gmail.com

 


Jurnal Ilmiah Kedokteran Wijaya Kusuma by https://journal.uwks.ac.id/index.php/jikw/index  is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.