Molecular Toxicology of Organophosphate Poisoning

Fery Setiawan, Arif Rahman Nurdianto, Heribertus Agustinus B Tena, Ahmad Yudianto, Jenny Sunariani, Achmad Basori, Acrivida Mega Charisma


The use of organophosphates (pesticides and other compounds to eradicate pests), currently, to increase the fulfillment of the population's consumption needs has a double-edged sword effect, on the one hand it can increase the need for food to be consumed by the population. The negative effect that can arise is the safety of organophosphate drugs which can contaminate the soil and water sources around the place where organophosphate drugs are used. The negative effects of organophosphates are associated with the effects of xenobiotics on humans who consume them. Xenobiotics are associated with toxicdynamic effects where organophosphates cause irreversible inhibition of the enzyme acetylcholinesterase (ACh). ACh is one of the main enzymes in the nervous system that terminates impulse conduction through the hydrolysis process of acetylcholine enzymes. Acetylcholinesterase is a specific molecular target of organophosphate pesticides. The inhibition of the Ach enzyme causes the inhibition of the acetylcholine enzyme which is normally always hydrolyzed by the Ach enzyme and is a specific biological marker of pesticide poisoning. Inhibition of ACh will cause the accumulation of the enzyme acetylcholine, resulting in negative effects of organophosphate poisoning which can lead to death. In this paper, the authors collect from various sources related to the study of molecular toxicology toxidynamic effects of drug safety and organophosphate poisoning. The results of this review article show that organophosphate poisoning is associated with irreversible inhibition of the acetylcholinesterase enzyme which results in death in the individual concerned.


Acetylcholinesterase; Drug Safety; Organophosphate; Toxicology; Xenobiotic

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Cedergreen N, Kristoffer D, and L, Michele G, Kretschmann AC, 2017. Can toxikokinetic and toxikodynamic modeling be used to understand and predict synergistic interaction between chemicals?. EST Washington. 1-12. DOI: 10.1021/acs.est.7b02723

David JP, Ismail HM, Proust AC, and Paine MJI, 2013. Role of cytochrome P450s in insecticide resistance: impact on control of mosquito-borne diseases and use of insecticides on Earth. Phil Trans R Soc B. 368: 1-12.

Dhamayanti FA and Shaftarina F, 2018. Efek Neurobehavioral akibat Paparan Kronik Organofosfat pada Petani. J Agromedicine. 5(1): 498-52

Eddleston M, 2019. Novel Clonical Toxicology and Pharmacology of Organophosphorus Insecticide Self-Poisoning. Annu. Rev. Pharmacol. Toxicol. 59: 341-60;

Fiananda AI, 2014. Hubungan Antara Aktivitas Asetilkolinesterase Darah dan Waktu Reaksi Petani Kentang dengan Paparan Kronik Pestisida Oganofosfat. Karya Tulis Ilmiah (KTI) Program Pendidikan Sarjana Kedokteran Fakultas Kedokteran Universitas Diponegoro Semarang.

Ghorab MA and Khalil MS, 2015. Toxicological Effects of Organophosphate Pesticides. IJEMA. 3(4): 218-20; doi: 10.11648/j.ijema.20150304.13

Hidayatullah T, Barliana MI, Pangaribuan B, Wijaya A, Sumiwi SA, Goenawan H, 2020. Hubungan Faktor Okupasi terhadap Aktivitas Asetilkolinesterase Eritrosit dan Fungsi Kognitif pada Petani yang Menggunakan Pestisida Organofosfat. Jurnal Farmasi Klinik Indonesia. 9(2): 128-136. DOI:10.15416/ijcp.2020.9.2.128

Hung DZ, Yang HJ, Li YF, Li CL, Chang SY, et al, 2015. The Long-Term Effects of Organophosphates Poisoning as a Risk Factor of CVDs: A Nationwide Population-Based Cohort Study. Plos One: 1-15; DOI:10.1371/journal.pone.0137632

Kamanyire R and Karalliedde L, 2004. Organophosphate toxicity and occupational exposure. Occmed. 54: 69-75. DOI: 10.1093/occmed/kqh018

Lenina OA, Zueva IV, Zobov VV, Semenov VE, Masson P, Petrov KA, 2020. Slow-binding reversible inhibitor of acetylcholinesterase with long-lasting action for prophylaxis of organophosphate poisoning. Scientific Reports. 10:16611;

Lionetto MG, Caricato R, Calisi A, Giordano ME, Schettino T.,2013. Acetylcholinesterase as a Biomarker in Environmental and Occupational Medicine: New Insights and Future Perspectives. BioMed Research International. 2013: 1-9;

Mladenovic M, Arsic BB, Stankovic N, Mihovic N, Ragno R, Regan A, Milicevic JS, Petrovic TMT, Micic R, 2020. The Targeted Pesticides as Acethylcholinesterase Inhibitors: Comprehensive Cross-Organism Molecular Modelling Studies Performed to Anticipate the Pharmacology of

Harmfulness to Humans In Vitro. MDPI Molecules. 23: 2192.


Paudyal BP, 2008. Organophosphorus Poisoning. J Nepal Med Assoc. 47(172): 251-8.

Perwitasari DA, Prasasti D, Supadmi W, Jaikishin SAD, Wiraagni IA, 2017. Impact of organophosphate exposure on farmer’ health in Kulon Progo, Yogyakarta: Perspectives of physical, emotional and social health. SAGE Open Medicine. 5: 1-6. DOI:10.1177/20501312117719092

Pittman J, 2007. Physiologically-Based Toxicokinetic and Toxicodynamic (PBTK/TD) Modeling of a Ternary Organophosphorus Insecticide Mixture in Rats: Model Development and Validation. Dissertation of Faculty of Mississippi State University.

Sinha PK and Sharma A, 2003. Organophosphat poisoning: A Review. Med J of Indones. 12(2): 120-127. DOI: 10.13181/mji.v12i2.100

Vermeire T, McPhail R, and Water M, 2010. Organophosphorus pesticides in the Environment. pp: 1-18.

Vicou VA, Thiermann H, Radulescu FS, Mircioiu C, Miron DS, 2009. Minireview The Toxicokinetics and Toxicodynamics of Organophosphates

versus Pharmakokinetics and Pharmakodynamics of Oxime Antidotes:

Biological Consequences. Basic & Clin Pharm and Toxicol. 106: 73-85.DOI: 10.1111/j.1742-7843.2009.00486.x

Wirasuta IMAG, 2008. Buku Ajar Analisis Toksikologi Forensik. Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana Bukit Jimbaran.



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