Efek Proteksi dari Terapi Oksigen Hiperbarik terhadap Ekspresi Bcl-2 Miometrium Rattus norvegicus Bunting yang Terinfeksi oleh Tachyzoite Toxoplasma gondii
Abstract
Hyperbaric Oxygen Therapy (HBOT) can increase oxygen delivery to tissues and stimulate the formation of H2O2 as a secondary messenger for phosphorylation of nuclear factor kappa beta (NF-kB) which plays an important role in the transcription of the anti apoptotic gene. This study aimed to determine the effects of Hyperbaric Oxygen Therapy (HBOT) in enhancing the expressions of Bcl-2 in the myometrium of pregnant rats infected by Toxoplasma gondii. This study was an experimental study with a randomized control group of post-test only and designed by 37 pregnant Rattus norvegicus Sprague Dawley. Randomly, the rats were divided into four groups. Group A is infected pregnant rats that exposed by 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group B is non-infected pregnant rats and exposed by 10 sessions of HBOT 2.4 ATA in 3x30 minutes. Group C is infected pregnant rats without any exposure. Group D is non-infected pregnant rats without any exposure. Each infected pregnant rat was given a 103 tachyzoite of T.gondii by intraperitoneal injection. Bcl-2 expressions were measured through immunohistochemistry. All data were analyzed using ANOVA test through SPSS 21 program application. There was a significant difference in Bcl-2 expression between Group A and Group C because p<α (p<0.017). HBOT can increase the expression of Bcl-2 from infected and not infected rat myometrium, in the provision of HBOT 2.4 ATA for 3x30 minutes, twice a day for 5 days.
Keywords
Full Text:
PDFReferences
Al-Fertosi RB and Juma ASM, 2006. Possible Cellular Expression of IFNγ in a Woman With Abortion Infected Toxoplasma Gondii. Medical Journal of Islamic World Academy of Sciences. 16(3): 121-134.
Barbosa BF, Lopes-Maria JB, Gomes AO, Angeloni MB, Castro AS et al, 2015. IL10, TGF Beta1, and IFN Gamma Modulate Intracellular Signaling Pathways and Cytokine Production to Control Toxoplasma gondii Infection in BeWo Trophoblast Cells. Biology Reproduction. 92(3): 82.
Bartlett Z, 2017. Apoptosis in Embryonic Development. Embryo Project Encyclopedia. ISSN: 1940-5030 http://embryo.asu.edu/handle/10776/11565.
Begum-Haque S, Haque A, Kasper LH, 2009. Apoptosis in Toxoplasma gondii Activated T cells: the role of IFNγ in Enhanced Alteration of Bcl-2 Expression and Mitochondrial Membrane Potential. Microb Pathog. 2009 November; 47(5): 281–288.
Clark DA, Coulam CB, Daya S, Chaouat G, 2001. Unexplained Sporadic and Recurrent Miscarriage in The New Millenium: A Critical Analysis of Immune Mechanisms and Treatments. Hum Reprod update. 7(5): 501-511.
Choudhury R, 2018. Hypoxia and hyperbaric oxygen therapy : a review. Int J Gem Med. 11: 431–442.
Danihel L, Gomolcak P, Korbel M, Pruzinec, J, Vojtaššak J et al, 2002. Expression of Proliferation and Apoptotic Markers in Human placenta During Pregnancy. Acta Histochem. 104(4): 335-338.
De Falco M, Fedele V, Cobellis L, Mastrogiacomo A, Leone S et al, 2004. Immunohistochemical Distribution of Proteins Belonging to The Receptor-Mediated and The Mitochondrial Apoptotic Pathways in Human Placenta During Gestation. Cell Tissue Res. 318(3): 599-608.
Dewi BDN, 2009. Efek Terapi Kombinasi Metformin-Oksigen Hyperbaric Pada Pasien Diabetes Mellitus Tipe 2: Peningkatan Kadar Endothelial Nitric Oxidesynthase (eNOS) dan Penurunan Kadar Glukosa Darah. Tesis. Surabaya. Universitas
Airlangga.
Dockrell DH, 2003. The Multiple Roles of Fas Ligand in The Pathogenesis of Infectious Diseases. Clin Microbiol Infect. 9(8): 766-779.
Ghasemi FS, Rasti S, Piroozmand A, Bandehpour M, Kazemi B et al 2016. Toxoplasmosis-Associated Abortion, and Stillbirth in Tehran, Iran. J Matern Fetal Neonatal Med. 29(2): 248-251.
Hill JA. 2004. Recurrent Pregnancy Loss. in: Creasy RK, Resnik R, editor. Maternal-Fetal Medicine Principle and Practice. 5th edition, Philadelphia: Saunders: 579-601.
Huppertz B, Kadyrov M, Kingdom JC, 2006. Apoptosis and Its Role in The Trophoblast. Am J Obstet Gynecol. 195(1): 29-39.
Jerzak M and Bischof P, 2002. Apoptosis in The First-Trimester Human Placenta: The Role in Maintaining Immune Privilege at The Maternal-Foetal Interface and in The Trophoblast Remodeling. Eur J Obstet Gynecol Reprod Biol. 100(2): 138-142.
Jeve YB and Davies W, 2014. Evidence-Based Management of Recurrent Miscarriages. J Hum Reprod Sci. 7(3): 159–169.
Kelly PN and Strasser A, 2011. The Role of Bcl-2 and Its Pro-Survival Relatives in Tumorigenesis and Cancer Therapy. Cell Death Differ. 18(9): 1414–1424.
Liu Z, Jiao QF, You C, Che YJ, Su FZ, 2006. Effect of Hyperbaric Oxygen on Cytochrome C, Bcl-2 and Bax Expression after Experimental Traumatic Brain Injury in Rats. Chin J Traumatol. 9(3): 168-174.
Moscrop A, 2013. ‘Miscarriage or abortion?’ Understanding the Medical Language of Pregnancy Loss in Britain; A Historical Perspective. Med Humanit. 39(2): 98–104.
Novak M, Madej JA, Dziegeil P, 2007. Intensity of Cox 2 expression in Cell of Soft Tissue Fibrosarcomas in Dogs as Related to Grade of Tumour Malignancy. Bull Vet inst Pulawy. 51, 275-279.
Nurdianto AR, Aryati, Suryokusumo MG, Mufasirin, 2019a. Effect of Hyperbaric Oxygen Therapy to IFNγ and TNFα Expression in Pregnant Rattus norvegicus Infected with Tachyzoite of Toxoplasma gondii. Bali Medical Journal; 8(1): 94 100.
Nurdianto AR, Aryati, Suryokusumo MG, Mufasirin, 2019b. Downregulates of ICAM1 expression in Myometrium from pregnant Rattus norvegicus infected with Tachyzoite of Toxoplasma gondii with Hyperbaric Oxygen Therapy. Hang Tuah Medical Journal; 17(1): 77-83
Opferman J and Kothari A, 2017. Anti-Apoptotic BCL-2 Family Members in Development. Cell Death and Differentiation. 25(1): 37–45.
Palzur E, Zaaroor M, Vlodavsky E, Milman F, Soustiel JF, 2008. Neuroprotective Effect of Hyperbaric Oxygen Therapy in Brain Injury is Mediated by Preservation of Mitochondrial Membrane Properties. Brain Res. 1221:126-133.
PrabhuDas M, Bonney E, Caron K, Dey S, Erlebacher A et al, 2015. Immune Mechanisms at the Maternal-Fetal Interface: Perspectives and Challenges. Nat Immunol. 16(4): 328–334.
Rusdiana D, 2014. Efek Oksigen Hyperbaric Terhadap Peningkatan Ekspresi Akt pada Rattus norvegicus dengan Diabetes Mellitus. Tesis. Surabaya. Universitas Airlangga.
Savion S, Lepsky E, Orenstein H, Carp H, Shepshelovich J et al, 2002. Apoptosis in the Uterus of Mice with Pregnancy Loss. Am J Reprod Immunol. 47(2): 118-127.
Sun QH, Peng JP, Xia HF, Yang Y. 2007. IFN-γ Promotes Apoptosis of the Uterus and Placenta in Pregnant Rat and Human Cytotrophoblast Cells. J Interferon Cytokine Res. 27(7): 567-578.
Sun Q and Zhang XL. 2017. Research on Apoptotic Signaling Pathways of A Recurrent Spontaneous Abortion Caused By Dysfunction of Trophoblast Infiltration. Eur Rev Med Pharmacol. 21(3 Suppl) : 12-19.
Toder V, Carp H, Fein A, Torchinsky A, 2002. The Role of Pro and Anti-Apoptotic Molecular Interactions in Embryonic Maldevelopment. Am J Reprod Immunol. 48(4): 235-244.
Torchinsky A and Toder V, 2004. To Die or Not to Die: The Function of the Transcription Factor NF-kB in Embryos Exposed to Stress. Am J Reprod Immunol. 51(2): 138-143.
Vlodavsky E, Palzur E, Soustiel JF, 2006. Hyperbaric Oxygen Therapy Reduces Neuroinflammation and Expression of Matrix Metalloproteinase-9 in The Rat Model of Traumatic Brain Injury.
Neuropathol Appl Neurobiol. 32(1): 40-50.
Weiss JL, Malone FD, Vidaver J, Ball RH, Nyberg DA et al, 2004. Threatened Abortion: A Risk Factor Poor Pregnancy Outcome, A Population-Based Screening Study. Am J Obstet Gynecol. 190(3): 745-750.
Xu S, Liu J, Zhang Y, Wang C, Wang J et al, 2012. Apoptosis-Related Protein Expression in Rabbits with Blast Brain Injury Following Early Hyperbaric Oxygen Therapy. Neural Regen Res. 7(17): 1318-1324.
DOI: http://dx.doi.org/10.30742/jikw.v9i1.730
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Arif Rahman Nurdianto
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Jurnal Ilmiah Kedokteran Wijaya Kusuma is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
